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http://hdl.handle.net/1783.1/1430
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| Title: | Temporal relationship between cytochrome c release and mitochondrial swelling during UV-induced apoptosis in living HeLa cells |
| Authors: | Gao, Wenhua
Pu, Yongmei
Luo, Kathy Q.
Chang, Donald C. |
| Keywords: | Cytochrome c
Apoptosis
Mitochondrial swelling
Green fluorescence protein
GFP
HeLa cells |
| Issue Date: | 2001 |
| Citation: | Journal of cell science, v. 114, no. 15, Aug. 2001, p. 2855-2862 |
| Abstract: | During apoptosis, cytochrome c is released from mitochondria to the cytosol to activate a caspase cascade, which commits the cell to the death process. It has been proposed that the release of cytochrome c is caused by a swelling of the mitochondrial matrix triggered by the apoptotic stimuli. To test this theory, we measured directly the dynamic re-distribution of green fluorescence protein (GFP)-tagged cytochrome c and morphological change of mitochondria within living HeLa cells during u.v.-induced apoptosis. We observed that mitochondria did not swell when cytochrome c was released from mitochondria to cytosol during apoptosis. Instead, mitochondria swelled to spherical shapes within 10 minutes of cytochrome c release. This finding strongly suggests that cytochrome c release in apoptosis was not caused by mitochondrial swelling. This conclusion was further supported in two separated experiments using an immunostaining method and carbonyl cyanide m-chlorophenyl-hydrazone (CCCP) treatment. In addition, we found evidence that cytochrome c was also released before mitochondrial swelling in apoptosis induced by other cell death-inducing treatments, including tumor necrosis factor (TNF) and actinomycin D. |
| Rights: | Permission is granted by the Company of Biologists Ltd. The paper was published in J Cell Sci 2001 114 : 2855-2862. |
| URI: | http://hdl.handle.net/1783.1/1430 |
| Appears in Collections: | BIOL Journal/Magazine Articles
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| 2855.pdf | | 1076Kb | Adobe PDF | View/Open |
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