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Title: Determination of sugar compounds in atmospheric aerosols by liquid chromatography combined with positive electrospray ionization mass spectrometry
Authors: Wan, Chun Hong
Yu, Jianzhen
Keywords: LC-MS analysis of aerosol organics
Sugar alcohols
Issue Date: Feb-2006
Citation: Journal of chromatography A, v. 1107, no. 1-2, Feb. 2006, p. 175-181
Abstract: We here report a method for the determination of sugar compounds of known presence in atmospheric aerosols using liquid chromatography (LC) combined with positive electrospray ionization mass spectrometry (MS). The target analytes include C3-C6 monosaccharide alcohols (glycerol, erythritol, xylitol, mannitol), C3-C6 monosaccharides (xylose, glucose, and 5 levoglucosan), a disaccharide (sucrose), and a trisaccharide (melezitose). A mobile phase consisting of 20% 10 mM aqueous ammonium acetate, 8% methanol, and 72% water was found to provide abundant [M+NH4]+ adduct ions when coupled with electrospray ionization. Use of a polymer-based amino analytical column resolved the target compounds from the bulk solvent and provided limited separation among the target compounds. The target 10 analytes were quantified using their [M+NH4]+ ions. Sample pretreatment was greatly simplified in comparison with the more commonly used gas chromatographic methods. It involved extraction of aerosol filters in methanol, evaporation of the solvent, and reconstitution with 5 mM ammonium acetate in water prior to the LC-MS analysis. The analyte recoveries were measured at the levels of 100, 500 and 1000 µg/L to be in the range 15 of 78–102%, 94–112%, and 92–110%, respectively. The detection limits were lower than 10 pmol/injection for the tested target compounds except for xylose. Xylose had a detection limit of 95 pmol/injection. The method was applied to analyze 30 atmospheric aerosol samples to demonstrate its feasibility. The LC-MS method made possible the detection of trisaccharides as aerosol constituents for the first time.
Rights: '[Journal of chromatography A] © copyright (2006) Elsevier. The Journal's web site is located at'
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