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Title: Biofunctional organic nanocrystals for quantitative detection of pathogen deoxyribonucleic acid
Authors: Chan, Cangel Pui Yee
Tzang, Lawrence Chi-hung
Sin, King-keung
Ji, Sheng-lin
Cheung, Kwan-yee
Tam, Tze-kin
Yang, Michael Meng-su
Renneberg, Reinhard
Seydack, Matthias
Keywords: Fluorescein diacetate (FDA)
Fluorescein 5-isothiocyanate (FITC)
Horseradish peroxidase (HRP)
Human papillomavirus (HPV)
Issue Date: Feb-2007
Citation: Analytica chimica acta, v. 584, no. 1, Feb. 2007, p. 7-11
Abstract: Advances in nanotechnology have had significant impacts on the field of biodiagnostics. In this study, we describe the novel application of dissolvable, organic, and biofunctional nanocrystals for the quantitative detection of a PCR product. Fluorescein diacetate (FDA), a fluorogenic precursor of fluorescein, was milled in a solution of a polymeric surfactant to create a stable, nanosized colloid with an interface for coupling streptavidin molecules. The application of these particulate labels for the quantitative detection of biotinylated human papillomavirus (HPV) DNA, amplified in a standard PCR procedure, was demonstrated. After the affinity reaction,the FDA molecules were dissolved and concomitantly converted into fluorescein. This approach resulted in a high selectivity, short incubation times and a sensitivity up to 147 times greater than obtained from state-of-the-art, directly fluorescent-labeled streptavidins. This innovative method offers rapid detection of small amounts of nucleic acids because less target material and thus fewer PCR cycles are required.
Rights: Analytica chimica acta © copyright (2007) Elsevier. The Journal's web site is located at
Appears in Collections:CHEM Journal/Magazine Articles

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