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http://hdl.handle.net/1783.1/2992
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| Title: | Development of a glucose-6-phosphate biosensor based on coimmobilized p-hydroxybenzoate hydroxylase and glucose-6-phosphate dehydrogenase |
| Authors: | Cui, Yue
Barford, John P.
Renneberg, Reinhard |
| Keywords: | Biosensor
Glucose-6-phosphate
p-Hydroxybenzoate hydroxylase
Glucose-6-phosphate dehydrogenase |
| Issue Date: | May-2007 |
| Citation: | Biosensors of bioelectronics, v. 22, no. 11, May 2007, p. 2754-2757 |
| Abstract: | This work reports the development of an amperometric glucose-6-phosphate biosensor by coimmobilizing p-hydroxybenzoate hydroxylase (HBH) and glucose-6-phosphate dehydrogenase (G6PDH) on a screen-printed electrode. The principle of the determination scheme is as follows: G6PDH catalyzes the specific dehydrogenation of glucose-6-phosphate by consuming NADP+. The product, NADPH, initiates the irreversible the hydroxylation of p-hydroxybenzoate by HBH in the presence of oxygen to produce 3, 4-dihydroxybenzoate, which results in a detectable signal due to its oxidation at the working electrode. The sensor shows a broad linear detection range between 2 μM and 1000 μM with a low detection limit of 1.2 μM. Also, it has a fast measuring time which can achieve 95% of the maximum current response in 20 s after the addition of a given concentration of glucose-6-phosphate with a short recovery time (2 min). |
| Rights: | Biosensors of bioelectronics © copyright (2007) Elsevier. The Journal's web site is located at http://www.sciencedirect.com/ |
| URI: | http://hdl.handle.net/1783.1/2992 |
| Appears in Collections: | CHEM Journal/Magazine Articles
CBME Journal/Magazine Articles
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| 2.Developmentofaglucose6phosphatebiosensorbasedoncoimmobilizedphydroxybenzoatehydroxylaseandglucose6phosphatedehydrogenase.pdf | pre-published version | 170Kb | Adobe PDF | View/Open |
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