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Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/6247
Title: Reporter gene assays
Authors: Liu, M. F.
New, D. C.
Lo, K. H.
Wong, Yung-Hou
Keywords: Reporter gene
Luciferase
Aequorin
GPCR
Receptor
G protein
Chimera
STAT
CRE
NFkB
Issue Date: 2009
Citation: Methods in molecular biology, v. 486, p. 109-123, 2009
Abstract: Reporter gene assays are versatile and sensitive methods of assaying numerous targets in high-throughput drug screening programs. A variety of reporter genes allow users a choice of signal that can be tailored to the required sensitivity, the available detection apparatus, the cellular system employed and the compatibility with multiplexed assays. Promoters used to drive the reporter gene expression can either be activated by a broad range of biochemical pathways or by the selective activation of individual targets. In this chapter, we will introduce some of the considerations behind the choice of reporter gene assays and describe the methods that we have used to establish 96-well format luciferase and aequorin assays for the screening of ligands for G protein-coupled receptors.
Rights: The original publication is available at http://www.springerlink.com/
URI: http://hdl.handle.net/1783.1/6247
Appears in Collections:BICH Journal/Magazine Articles

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