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|Title: ||Battery-powered miniaturized electroporation system for in-situ gene transfer in stem cell research|
|Authors: ||He, Huiqi|
Chang, Donald C.
In-situ gene transfer
|Issue Date: ||Jan-2009 |
|Citation: ||Proceedings of the 2009 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, January 5-8, 2009, Shenzhen, China, p. 463-467.|
|Abstract: ||A battery-powered miniaturized electroporation (EP) system was designed and fabricated to tackle the challenging DNA transfection of stem cells. The system consists of a programmable mini-electroporator and a micro EP chip. Large statistical data of loading efficiency for five different size molecules and cell viability were determined under various voltage and duration in this system for HeLa cell and MEF cell line. Based on more than 2,000 single-cell electroporation experiments, an EP phase diagram for MEF cells have been generated for the first time. It can be used to significantly increase the in-situ gene transfer efficiency. In addition, the EP boundary curve for MEF cells can be described by a simple empirical formula. Comparing to the conventional instrument, the miniaturized electroporation system can greatly shorten the experimental time. We also proposed a new system for continuous online monitoring in-situ gene expression, which is still in progress.|
|Rights: ||© 2009 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.|
|Appears in Collections:||MECH Conference Papers|
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