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Title: A prototypic microfluidic platform generating stepwise concentration gradients for real-time study of cell apoptosis
Authors: Dai, Wen
Zheng, Yizhe
Luo, Kathy Q.
Wu, Hongkai
Keywords: Biomedical measurement
Cellular biophysics
Fluorescence spectroscopy
Optical microscopy
Issue Date: Jun-2010
Citation: Biomicrofluidics v.4, iss. 2, 024101
Abstract: This work describes the development of a prototypic microfluidic platform for the generation of stepwise concentration gradients of drugs. A sensitive apoptotic analysis method is integrated into this microfluidic system for studying apoptosis of HeLa cells under the influence of anticancer drug, etoposide, with various concentrations in parallel; it measures the yellow fluorescent protein/cyan fluorescent protein fluorescence resonance energy transfer (FRET) signal that responds to the activation of caspase-3, an indicator of cell apoptosis. Sets of microfluidic valves on the chip generate stepwise concentration gradient of etoposide in various cell-culture microchambers. The FRET signals from multiple chambers are simultaneously monitored under a fluorescent microscope for long-time observation and the on-chip results are compared with those from 96-well plate study and the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The microfluidic platform shows several advantages including high-throughput capacity, low drug consumption, and high sensitivity.
Rights: Copyright 2010 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Biomicrofluidics and may be found at
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