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Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/7280
Title: Axonal filopodial asymmetry induced by synaptic target
Authors: Li, Pan
Chen, Gheng
Lee, Chi Wai
Madhavan, Raghavan
Peng, H. Benjamin
Keywords: Cell interaction
Neuromuscular junction
Neuronal communication
Issue Date: 15-Jul-2011
Citation: Molecular biology of the cell, v. 22, no. 14, 2011
Abstract: ABSTRACT During vertebrate neuromuscular junction (NMJ) assembly, motor axons and their muscle targets exchange short-range signals that regulate the subsequent steps of presynaptic and postsynaptic specialization. We report here that this interaction is in part mediated by axonal filopodia extended preferentially by cultured Xenopus spinal neurons toward their muscle targets. Immunoblotting and labeling experiments showed that basic fibroblast growth factor (bFGF) was expressed by muscle and associated with the cell surface, and treatment of cultured spinal neurons with recombinant bFGF nearly doubled the normal density of filopodia in neurites. This effect of bFGF was abolished by SU5402, a selective inhibitor of FGF-receptor 1 (FGFR1), and forced expression of wild-type or dominant-negative FGFR1 in neurons enhanced or suppressed the assembly of filopodia, respectively. Significantly, in nerve–muscle cocultures, knocking down bFGF in muscle decreased both the asymmetric extension of filopodia by axons toward muscle and the assembly of NMJs. In addition, neurons expressing dominant-negative FGFR1 less effectively triggered the aggregation of muscle acetylcholine receptors at innervation sites than did control neurons. These results suggest that bFGF activation of neuronal FGFR1 generates filopodial processes in neurons that promote nerve–muscle interaction and facilitate NMJ establishment.
Rights: Copyright © 2011 The Amercian Society for Cell Biology
URI: http://hdl.handle.net/1783.1/7280
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