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Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/741
Title: Deformation of DNA molecules by hydrodynamic focusing
Authors: Lee, Yi-Kuen
Keywords: Single DNA dynamics
Microfluidics
Issue Date: Dec-2003
Citation: Journal of fluid mechanics, vol. 497, Dec. 2003, p. 55-65
Abstract: The motion of a DNA molecule in a solvent flow reflects the deformation of a nano/micro scale flexible mass-spring structure by the forces exerted by the fluid molecules. The dynamics of individual molecules can reveal both fundamental properties of the DNA and basic understandings of the complex rheological properties of long chain molecules. In this study, we report the dynamic of isolated DNA molecules under homogeneous extensional flow. Hydrodynamic focusing is able to generate homogeneous extensional flow with uniform velocity in the transverse direction. The deformation of individual DNA molecules in the flow was visualized with video fluorescence microscopy. A coil-stretch transition was observed when the Deborah number (De) is larger than 0.8. With a sudden stop of the flow, the DNA molecule relaxes and recoils. Longest relaxation time of T2 DNA was determined to be 0.63s when scaling viscosity to 0.9cP, which is in agreement with values obtained with other methods.
Rights: © Cambridge University Press 2003. This paper was published in Journal of Fluid Mechanics, vol. 497, Dec. 2003, pp. 55-65 and is reprinted with permission.
URI: http://hdl.handle.net/1783.1/741
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