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Salmonella enterica serovar Typhi uses type IVB pili to enter human intestinal epithelial cells

Authors Zhang, XL
Tsui, ISM
Yip, CMC
Fung, AWY
Wong, DKH
Dai, XY
Yang, YH
Hackett, J
Morris, C
Issue Date 2000
Source Infection and immunity , v. 68, (6), 2000, JUN, p. 3067-3073
Summary DNA sequencing upstream of the Salmonella enterica serovar Typhi pilV and rci genes previously identified in the ca, 118-kb major pathogenicity island (X.-L. Zhang, C. Morris, and J. Hackett, Gene 202:139-146, 1997) identified a further 10 pil genes apparently forming a pil operon, The product of the pilS gene, prePilS protein (a putative type IVB structural prepilin) was purified, and an anti-prePilS antiserum was raised in mice. Mutants of serovar Typhi either lacking the whole pil operon or with an insertion mutation in the pilS gene were constructed, as was a strain in which the pilN to pilV genes were driven by the tac promoter. The pil(+) strains synthesized type IVB pill, as judged by (i) visualization in the electron microscope of thin pill in culture supernatants of one such strain and (ii) the presence of PilS protein (smaller than the prePilS protein by removal of the leader peptide) on immunoblotting of material pelleted by high-speed centrifugation of either the culture supernatant or sonicates of pil(+) strains. Control pil mutants did not express the PilS protein. A pilS mutant of serovar Typhi entered human intestinal INT407 cells in culture to levels only 5 to 25\% of those of the wild-type strain, and serovar Typhi entry was strongly inhibited by soluble prePilS protein (50\% inhibition of entry at 1.4 mu M prePilS).
ISSN 0019-9567
Rights Copyright © American Society for Microbiology [Infection and Immunity, v. 68, no. 6, June 2000, p. 3067-3073]
Language English
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