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Temporal relationship between cytochrome c release and mitochondrial swelling during UV-induced apoptosis in living HeLa cells

Authors Gao, WH
Pu, YM HKUST affiliated (currently or previously)
Luo, KQ HKUST affiliated (currently or previously)
Chang, DC HKUST affiliated (currently or previously)
Issue Date 2001
Source Journal of cell science , v. 114, (15), 2001, AUG, p. 2855-2862
Summary During apoptosis, cytochrome c is released from mitochondria to the cytosol to activate a caspase cascade, which commits the cell to the death process. It has been proposed that the release of cytochrome c is caused by a swelling of the mitochondrial matrix triggered by the apoptotic stimuli. To test this theory, we measured directly the dynamic re-distribution of green fluorescence protein (GFP)-tagged cytochrome c and morphological change of mitochondria within living HeLa cells during u.v.-induced apoptosis. We observed that mitochondria did not swell when cytochrome c was released from mitochondria to cytosol during apoptosis. Instead, mitochondria swelled to spherical shapes within 10 minutes of cytochrome c release. This finding strongly suggests that cytochrome c release in apoptosis was not caused by mitochondrial swelling. This conclusion was further supported in two separated experiments using an immunostaining method and carbonyl cyanide m-chlorophenyl-hydrazone (CCCP) treatment. In addition, we found evidence that cytochrome c was also released before mitochondrial swelling in apoptosis induced by other cell death-inducing treatments, including tumor necrosis factor (TNF) and actinomycin D.
ISSN 0021-9533
Rights Permission is granted by the Company of Biologists Ltd. The paper was published in J Cell Sci 2001 114 : 2855-2862.
Language English
Format Article
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