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Rapid Quantification of the Clinically Important Analytes in Sub-Mu l Simulating Human Sera

Authors Qu, Jianan View this author's profile
Shao, Lan HKUST affiliated (currently or previously).
Issue Date 2000
Source Proceedings of SPIE - The International Society for Optical Engineering , v. 3918, 2000, p. 174-180
Summary Near infrared Raman spectroscopy has been successfully used to analyze major metabolites in simulating human serum samples quantitatively. We demonstrated that the optical waveguide based Raman cell and signal collector can significantly improve the collection efficiency of inherently weak Raman signal. The acquisition time for the Raman signal with decent SNR has been reduced to 10 seconds. The sample container, a quartz capillary with length of 20 mm and diameter of 400 mu m, is easy to produce and disposable. The Raman signal was normalized to the dominant water peak at 3350 cm(-1). This self-calibration method makes the measurement insensitive to the fluctuation of the excitation and misalignment of the collection system. A partial least squares method was used to predict the analyte concentrations of interest in the simulating human sera. The prediction accuracy of albumin, globulin, triactin, urea and glucose is greatly acceptable for clinical diagnostics. The results of this research encourage the development of practical Raman system for clinical diagnostics.
Note Biomedical Spectroscopy: Vibrational Spectroscopy and other Novel Techniques; San Jose, CA, USA; 26 January 2000 through 27 January 2000; Code 57010
ISSN 0277-786X
ISBN 0819435341
Rights Copyright 2000 Society of Photo-Optical Instrumentation Engineers. This paper was published in Biomedical Spectroscopy : Vibrational Spectroscopy and Other Novel Techniques, Anita Mahadevan-Jansen, Gerwin J. Puppels, Editors, Proceedings of SPIE Vol. 3918, p. 174-180 (2000) and is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Language English
Format Conference paper
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