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Combination of Diffuse-Reflectance and Fluorescence Imaging of Turbid Media

Authors Qu, Jianan Y. View this author's profile
Hua, Jianwen HKUST affiliated (currently or previously).
Huang, Zhijian HKUST affiliated (currently or previously).
Issue Date 2000
Source Proceedings of SPIE - The International Society for Optical Engineering , v. 3917, 2000, p. 56-61
Summary We have used the cross-polarization method to eliminate the specular reflection and enhance the diffusive back-scattering of polarized fluorescence excitation light from the turbid media. The image of the ratio between fluorescence and cross-polarized reflection provides a map of the fluorescence yield, defined as the ratio of the autofluorescence emission to the illumination incidence, over the surface of the turbid media when excitation and collection of fluorescence are highly inhomogeneous. The simple ratio imaging technique shows the feasibility to detect early cancer, which usually starts from the superficial layer of tissue, based on the contrast in the fluorescence yield between lesion and normal tissue.
Note Optical Biopsy III; San Jose, CA, USA; 23 January 2000 through 24 January 2000; Code 56967
ISSN 0277-786X
ISBN 0819435333
Rights Copyright 2000 Society of Photo-Optical Instrumentation Engineers. This paper was published in Optical Biopsy III, Robert R. Alfano, Editor, Proceedings of SPIE Vol. 3917, p. 56-61 (2000) and is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Language English
Format Conference paper
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