Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/2649

Determination of sugar compounds in atmospheric aerosols by liquid chromatography combined with positive electrospray ionization mass spectrometry

Authors Wan, ECH
Yu, JZ
Issue Date 2006
Source Journal of chromatography A, v. 1107, (1-2), 2006, FEB 24, p. 175-181
Summary We here report a method for the determination of sugar compounds of known presence in atmospheric aerosols using liquid chromatography (LC) combined with positive electrospray ionization mass spectrometry (MS). The target analytes include C-3-C-6 monosaccharide alcohols (glycerol, erythritol, xylitol, mannitol), C-5-C-6 monosaccharides (xylose, glucose, and levoglucosan), a disaccharide (sucrose), and a trisaccharide (melezitose). A mobile phase consisting of 20\% 10 mM aqueous ammonium acetate, 8\% methanol, and 72\% water was found to provide abundant [M + NH4](+) adduct ions when coupled with electrospray ionization. Use of a polymer-based amino analytical column resolved the target compounds from the bulk solvent and provided limited separation among the target compounds. The tat-get analytes were quantified using their [M + NH4](+) ions. Sample pretreatment was greatly simplified in comparison with the more commonly used gas chromatographic methods. It involved extraction of aerosol filters in methanol, evaporation of the solvent, and reconstitution with 5 mM ammonium acetate in water prior to the LC-MS analysis. The analyte recoveries were measured at the levels of 100, 500 and 1000 mu g/L to be in the range of 78-102\%, 94-112\%, and 92-110\%, respectively. The detection limits were lower than 10 pmol/injection for the tested target compounds except for xylose. Xylose had a detection limit of 95 pmol/injection. The method was applied to analyze 30 atmospheric aerosol samples to demonstrate its feasibility. The LC-MS method made possible the detection of trisaccharides as aerosol constituents for the first time. (c) 2005 Elsevier B.V. All rights reserved.
Subjects
ISSN 0021-9673
Rights '[Journal of chromatography A] © copyright (2006) Elsevier. The Journal's web site is located at http://www.sciencedirect.com/'
Language English
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