Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/2649

Determination of sugar compounds in atmospheric aerosols by liquid chromatography combined with positive electrospray ionization mass spectrometry

Authors Wan, ECH
Yu, JZ
Issue Date 2006
Source Journal of chromatography A , v. 1107, (1-2), 2006, FEB 24, p. 175-181
Summary We here report a method for the determination of sugar compounds of known presence in atmospheric aerosols using liquid chromatography (LC) combined with positive electrospray ionization mass spectrometry (MS). The target analytes include C-3-C-6 monosaccharide alcohols (glycerol, erythritol, xylitol, mannitol), C-5-C-6 monosaccharides (xylose, glucose, and levoglucosan), a disaccharide (sucrose), and a trisaccharide (melezitose). A mobile phase consisting of 20\% 10 mM aqueous ammonium acetate, 8\% methanol, and 72\% water was found to provide abundant [M + NH4](+) adduct ions when coupled with electrospray ionization. Use of a polymer-based amino analytical column resolved the target compounds from the bulk solvent and provided limited separation among the target compounds. The tat-get analytes were quantified using their [M + NH4](+) ions. Sample pretreatment was greatly simplified in comparison with the more commonly used gas chromatographic methods. It involved extraction of aerosol filters in methanol, evaporation of the solvent, and reconstitution with 5 mM ammonium acetate in water prior to the LC-MS analysis. The analyte recoveries were measured at the levels of 100, 500 and 1000 mu g/L to be in the range of 78-102\%, 94-112\%, and 92-110\%, respectively. The detection limits were lower than 10 pmol/injection for the tested target compounds except for xylose. Xylose had a detection limit of 95 pmol/injection. The method was applied to analyze 30 atmospheric aerosol samples to demonstrate its feasibility. The LC-MS method made possible the detection of trisaccharides as aerosol constituents for the first time. (c) 2005 Elsevier B.V. All rights reserved.
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ISSN 0021-9673
Rights '[Journal of chromatography A] © copyright (2006) Elsevier. The Journal's web site is located at http://www.sciencedirect.com/'
Language English
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