Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/2650

Type II topoisomerase activities in both the G(1) and G(2)/M phases of the dinoflagellate cell cycle

Authors Mak, CKM
Hung, VKL
Wong, JTY
Issue Date 2005
Source Chromosoma , v. 114, (6), 2005, DEC, p. 420-431
Summary Dinoflagellate genomes are large (up to 200 pg) and are encoded in histoneless chromosomes that are quasi-permanently condensed. This unique combination of chromosomal characteristics presents additional topological and cell cycle control problems for a eukaryotic cell, potentially exhibiting novel regulatory requirements of topoisomerase II. The heterotrophic dinoflagellate Crypthecodinium cohnii was used in this study. The topoisomerase II activities throughout its cell cycle were investigated by DNA flow cytometry following enzyme deactivation. Fluorescence microscopy was also used for studying the chromosome morphology of the treated cells. Two classes of topoisomerase II inhibitors were applied in our study, both of which caused G(1) delay as well as G(2)/M arrest in the C. cohnii cell cycle. At high doses, the topoisomerase poisons amsacrine and ellipticine induced DNA fragmentation in C. cohnii cells. Topoisomerase II activities, as measured by the ability to decatenate kinetoplastid DNA (kDNA), are normally detected throughout the cell cycle in C. cohnii. Our results suggest that the requirement of type II topoisomerase activities during the G(1) phase of the cell cycle may relate to the unwinding of quasi-permanently condensed chromosomes for the purpose of transcription. This was also the first time that topoisomerase II activity in dinoflagellate cells was detected.
Subjects
ISSN 0009-5915
Rights The original publication is available at http://www.springerlink.com/content/up3925t6n4u7325j
Language English
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