Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/2991

Biofunctional organic nanocrystals for quantitative detection of pathogen deoxyribonucleic acid

Authors Chan, Cangel Pui-yee
Tzang, Lawrence Chi-hung
Sin, King-keung
Ji, Sheng-lin
Cheung, Kwan-yee
Tam, Tze-kin
Yang, Michael Meng-su
Renneberg, Reinhard
Seydack, Matthias
Issue Date 2007
Source Analytica Chimica Acta , v. 584, (1), 2007, FEB 12, p. 7-11
Summary Advances in nanotechnology have had significant impacts in the field of biodiagnostics. In this study, we describe the novel application of dissolvable, organic and biofunctional nanocrystals for the quantitative detection of a PCR product. Fluorescein diacetate (FDA), a fluorogenic precursor of fluorescein, was milled in a solution of a polymeric surfactant to create a stable, nanosized colloid with an interface for coupling streptavidin molecules. The application of these particulate labels for the quantitative detection of biotinylated human papillomavirus (HPV) DNA, amplified in a standard PCR procedure, was demonstrated. After the affinity reaction, the FDA molecules were dissolved and concomitantly converted into fluorescein. This approach resulted in a high selectivity, short incubation times and a sensitivity up to 147 times greater than obtained from state-of-the-art, directly fluorescent-labeled streptavidins. This innovative method offers rapid detection of small amounts of nucleic acids because less target material and thus fewer PCR cycles are required. (c) 2006 Elsevier B.V. All rights reserved.
Subjects
ISSN 0003-2670
Rights Analytica chimica acta © copyright (2007) Elsevier. The Journal's web site is located at http://www.sciencedirect.com/
Language English
Format Article
Access View full-text via DOI
View full-text via Web of Science
View full-text via Scopus
Find@HKUST
Files in this item:
File Description Size Format
ACA061705Rev.pdf 116499 B Adobe PDF