Please use this identifier to cite or link to this item: http://hdl.handle.net/1783.1/2992

Development of a glucose-6-phosphate biosensor based on coimmobilized p-hydroxybenzoate hydroxylase and glucose-6-phosphate dehydrogenase

Authors Cui, Yue
Barford, John P.
Renneberg, Reinhard
Issue Date 2007
Source Biosensors & bioelectronics , v. 22, (11), 2007, MAY 15, p. 2754-2758
Summary This work reports the development of an amperometric glucose-6-phosphate biosensor by coimmobilizing p-hydroxybenzoate hydroxylase (HBH) and glucose-6-phosphate dehydrogenase (G6PDH) on a screen-printed electrode. The principle of the determination scheme is as follows: G6PDH catalyzes the specific dehydrogenation of glucose-6-phosphate by consuming NADP(+). The product, NADPH, initiates the irreversible the hydroxylation of p-hydroxybenzoate by HBH in the presence of oxygen to produce 3,4-dihydroxybenzoate, which results in a detectable signal due to its oxidation at the working electrode. The sensor shows a broad linear detection range between 2 mu M and 1000 mu M with a low detection limit of 1.2 mu M. Also, it has a fast measuring time which can achieve 95\% of the maximum current response in 20 s after the addition of a given concentration of glucose-6-phosphate with a short recovery time (2 min). (C) 2006 Elsevier B.V. All rights reserved.
Subjects
ISSN 0956-5663
Rights Biosensors of bioelectronics © copyright (2007) Elsevier. The Journal's web site is located at http://www.sciencedirect.com/
Language English
Format Article
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