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Alteration of Xylose Reductase Coenzyme Preference to Improve Ethanol Production by Saccharomyces Cerevisiae from High Xylose Concentrations

Authors Xiong, Mingyong
Chen, Guohua View this author's profile
Barford, John View this author's profile
Issue Date 2011
Source Bioresource technology , v. 102, (19), October 2011, p. 9206-9215
Summary A K270R mutation of xylose reductase (XR) was constructed by site-direct mutagenesis. Fermentation results of the F106X and F106KR strains, which carried wild type XR and K270R respectively, were compared using different substrate concentrations (from 55 to 220 g/L). After 72 h, F106X produced less ethanol than xylitol, while F106KR produced ethanol at a constant yield of 0.36 g/g for all xylose concentrations. The xylose consumption rate and ethanol productivity increased with increasing xylose concentrations in F106KR strain. In particular, F106KR produced 77.6 g/L ethanol from 220 g/L xylose and converted 100 g/L glucose and 100 g/L xylose into 89.0 g/L ethanol in 72 h, but the corresponding values of F106X strain are 7.5 and 65.8 g/L. The ethanol yield of F106KR from glucose and xylose was 0.42 g/g, which was 82.3% of the theoretical yield. These results suggest that the F106KR strain is an excellent producer of ethanol from xylose. (C) 2011 Elsevier Ltd. All rights reserved.
ISSN 0960-8524
Language English
Format Article
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