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The function of mitochondria in presynaptic development at the neuromuscular junction

Authors Lee, Chi Wai HKUST affiliated (currently or previously)
Peng, H. Benjamin View this author's profile
Issue Date 2008
Source Molecular biology of the cell , v. 19, (1), 2008, JAN, p. 150-158
Summary Mitochondria with high membrane potential (Delta Psi(m)) are enriched in the presynaptic nerve terminal at vertebrate neuromuscular junctions, but the exact function of these localized synaptic mitochondria remains unclear. Here, we investigated the correlation between mitochondrial Delta Psi(m) and the development of synaptic specializations. Using mitochondrial Delta Psi(m)-sensitive probe JC-1, we found that Delta Psi(m) in Xenopus spinal neurons could be reversibly elevated by creatine and suppressed by FCCP. Along naive neurites, preexisting synaptic vesicle (SV) clusters were positively correlated with mitochondrial Delta Psi(m), suggesting a potential regulatory role of mitochondrial activity in synaptogenesis. Indicating a specific role of mitochondrial activity in presynaptic development, mitochondrial ATP synthase inhibitor oligomycin, but not mitochondrial Na+/Ca2+ exchanger inhibitor CGP-37157, inhibited the clustering of SVs induced by growth factor-coated beads. Local F-actin assembly induced along spinal neurites by beads was suppressed by FCCP or oligomycin. Our results suggest that a key role of presynaptic mitochondria is to provide ATP for the assembly of actin cytoskeleton involved in the assembly of the presynaptic specialization including the clustering of SVs and mitochondria themselves.
ISSN 1059-1524
Rights The American Society for Cell Biology is the copyright holder for this article
Language English
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