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Identification of CD8+ T cell epitopes from HCA661 presented by HLA-A2 molecules

Authors Pang, Ha Sang
Issue Date 2006
Summary In this project, I used reverse immunology to identify the peptide HCA661246-254 as the dominant epitope of HCA661, a newly identified CT antigen presented by HLA molecules in hepatocellular carcinoma (HCC) patients. HCA661246-254 was able to generate peptide-specific T cells which recognized HCA66+/HLA-A2+ cancer cell line HepG2. Results of LDH cytotoxicity and ELISPOT assays confirmed that HCA661246-254 was able to activate T cells specifically targeting HepG2 cells. Intracellular IFN-γ secretion assay further confirmed the targeting activity of HCA661246-254-specific T cells. The results of this study indicate that HCA661246-254 is the most frequently presented peptide by cancer cells on HLA-A2 molecules. HCA661246-254 may be a good candidate for clinical analysis.
Note Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2006
Language English
Format Thesis
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