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An amine-reactive tetraphenylethylene derivative for protein detection in SDS-PAGE

Authors Yu, Yong HKUST affiliated (currently or previously)
Qin, Anjun HKUST affiliated (currently or previously)
Feng, Chao HKUST affiliated (currently or previously)
Lu, Ping
Ng, Ka Ming View this author's profile
Luo, Kathy Qian
Tang, Benzhong View this author's profile
Issue Date 2012
Source Analyst , v. 137, (23), 2012, p. 5592-5596
Summary A new aggregation-induced emission (AIE) compound 1,2-bis[4-(isothiocyanatomethyl)phenyl]-1,2-diphenylethene (2) was synthesized for use in SDS-PAGE. The molecule is practically nonemissive in solution but becomes highly emissive after reacting with the amine groups of the proteins by either the prestaining or poststaining method. The sensitivity of 2 achieved in the prestaining method is the same as that of Coomassie brilliant blue (CBB), while that observed in the poststaining method is higher than that of CBB. Excellent linear responses with the amount of protein were obtained in both cases. The detection of a mixture of proteins with different molecular weights was successfully achieved.
ISSN 0003-2654
Language English
Format Article
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