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Active mask segmentation of fluorescence images

Authors Srinivasa, Gowri
Fickus, Matthew C.
Guo, Yusong View this author's profile
Linstedt, Adam D.
Kovačević, Jelena V.
Issue Date 2009
Source 5th IEEE International Symposium on Biomedical Imaging, Paris, FRANCE, 14-17 May 2008, IEEE Transactions on Image Processing , v. 18, Issue 8, 2009, p. 1817-1829
Summary We propose a new active mask algorithm for the segmentation of fluorescence microscope images of punctate patterns. It combines the (a) flexibility offered by active-contour methods, (b) speed offered by multiresolution methods, (c) smoothing offered by multiscale methods, and (d) statistical modeling offered by region-growing methods into a fast and accurate segmentation tool. The framework moves from the idea of the "contour" to that of "inside and outside," or masks, allowing for easy multidimensional segmentation. It adapts to the topology of the image through the use of multiple masks. The algorithm is almost invariant under initialization, allowing for random initialization, and uses a few easily tunable parameters. Experiments show that the active mask algorithm matches the ground truth well and outperforms the algorithm widely used in fluorescence microscopy, seeded watershed, both qualitatively, as well as quantitatively.
ISSN 10577149
Language English
Format Conference paper
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