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A Prototypic Microfluidic Platform Generating Stepwise Concentration Gradients for Real-time Study of Cell Apoptosis

Authors Dai, Wen
Zheng, Yizhe HKUST affiliated (currently or previously)
Luo, Kathy Qian HKUST affiliated (currently or previously)
Wu, Hongkai View this author's profile
Issue Date 2010
Source Biomicrofluidics , v. 4, (2), 2010, JUN
Summary This work describes the development of a prototypic microfluidic platform for the generation of stepwise concentration gradients of drugs. A sensitive apoptotic analysis method is integrated into this microfluidic system for studying apoptosis of HeLa cells under the influence of anticancer drug, etoposide, with various concentrations in parallel; it measures the yellow fluorescent protein/cyan fluorescent protein fluorescence resonance energy transfer (FRET) signal that responds to the activation of caspase-3, an indicator of cell apoptosis. Sets of microfluidic valves on the chip generate stepwise concentration gradient of etoposide in various cell-culture microchambers. The FRET signals from multiple chambers are simultaneously monitored under a fluorescent microscope for long-time observation and the on-chip results are compared with those from 96-well plate study and the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The microfluidic platform shows several advantages including high-throughput capacity, low drug consumption, and high sensitivity. (C) 2010 American Institute of Physics. [doi:10.1063/1.3398319]
ISSN 1932-1058
Rights Copyright 2010 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Biomicrofluidics and may be found at
Language English
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