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Deformation of DNA molecules by hydrodynamic focusing

Authors Wong, Pakkin
Lee, Yi-Kuen View this author's profile
Ho, Chih-Ming
Issue Date 2003
Source Journal of fluid mechanics , v. 497, 2003, DEC 25, p. 55-65
Summary The motion of a DNA molecule in a solvent flow reflects the deformation of a nano/microscale flexible mass-spring structure by the forces exerted by the fluid molecules. The dynamics of individual molecules can reveal both fundamental properties of the DNA and basic understanding of the complex rheological properties of long-chain molecules. In this study, we report the dynamics of isolated DNA molecules under homogeneous extensional flow. Hydrodynamic focusing generates homogeneous extensional flow with uniform velocity in the transverse direction. The deformation of individual DNA molecules in the flow was visualized with video fluorescence microscopy. A coil-stretch transition was observed when the Deborah number (De) is larger than 0.8. With a sudden stopping of the flow, the DNA molecule relaxes and recoils. The longest relaxation time of T2 DNA was determined to be 0.63 s when scaling viscosity to 0.9 cP.
ISSN 0022-1120
Rights © Cambridge University Press 2003. This paper was published in Journal of Fluid Mechanics, vol. 497, Dec. 2003, pp. 55-65 and is reprinted with permission.
Language English
Format Article
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