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B12-Dependent Photoresponsive Protein Hydrogels for Controlled Stem Cell/Protein Release

Authors Wang, Ri HKUST affiliated (currently or previously)
Yang, Zhongguang HKUST affiliated (currently or previously)
Luo, Jiren HKUST affiliated (currently or previously)
Hsing, I-Ming View this author's profile
Sun, Fei View this author's profile
Issue Date 2017
Source Proceedings of the National Academy of Sciences of the United States of America , v. 114, (23), June 2017, p. 5912-5917
Summary Thanks to the precise control over their structural and functional properties, genetically engineered protein-based hydrogels have emerged as a promising candidate for biomedical applications. Given the growing demand for creating stimuli-responsive “smart” hydrogels, here we show the synthesis of entirely protein-based photoresponsive hydrogels by covalently polymerizing the adenosylcobalamin (AdoB12)-dependent photoreceptor C-terminal adenosylcobalamin binding domain (CarHC) proteins using genetically encoded SpyTag-SpyCatcher chemistry under mild physiological conditions. The resulting hydrogel composed of physically self-assembled CarHC polymers exhibited a rapid gel-sol transition on light exposure, which enabled the facile release/recovery of 3T3 fibroblasts and human mesenchymal stem cells (hMSCs) from 3D cultures while maintaining their viability. A covalently cross-linked CarHC hydrogel was also designed to encapsulate and release bulky globular proteins, such as mCherry, in a light-dependent manner. The direct assembly of stimuli-responsive proteins into hydrogels represents a versatile strategy for designing dynamically tunable materials.
ISSN 0027-8424
Language English
Format Article
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