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Apical Constriction is Driven by a Pulsatile Apical Myosin Network in Delaminating Drosophila Neuroblasts

Authors An, Yanru HKUST affiliated (currently or previously)
Xue, Guosheng HKUST affiliated (currently or previously).
Yang, Shaobo HKUST affiliated (currently or previously)
Deng, Mingxi HKUST affiliated (currently or previously)
Zhou, Xiaowei HKUST affiliated (currently or previously)
Yu, Weichuan View this author's profile
Ishibashi, Toyotaka View this author's profile
Zhang, Lei
Yan, Yan View this author's profile
Issue Date 2017
Source Journal of Cell Science , v. 130, (13), 2017, p. 2153-2164
Summary Cell delamination is a conserved morphogenetic process important for the generation of cell diversity and maintenance of tissue homeostasis. Here, we used Drosophila embryonic neuroblasts as a model to study the apical constriction process during cell delamination. We observe dynamic myosin signals both around the cell adherens junctions and underneath the cell apical surface in the neuroectoderm. On the cell apical cortex, the nonjunctional myosin forms flows and pulses, which are termed medial myosin pulses. Quantitative differences in medial myosin pulse intensity and frequency are crucial to distinguish delaminating neuroblasts from their neighbors. Inhibition of medial myosin pulses blocks delamination. The fate of a neuroblast is set apart from that of its neighbors by Notch signaling-mediated lateral inhibition. When we inhibit Notch signaling activity in the embryo, we observe that small clusters of cells undergo apical constriction and display an abnormal apical myosin pattern. Together, these results demonstrate that a contractile actomyosin network across the apical cell surface is organized to drive apical constriction in delaminating neuroblasts. © 2017. Published by The Company of Biologists Ltd.
ISSN 0021-9533
Language English
Format Article
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